The dietary supplement 5-hydroxytryptophan and urinary 5-hydroxyindole acetic acid

The dietary supplement 5-hydroxytryptophan and urinary 5-hydroxyindole acetic acid.

Colorimetric vs liquid-chromatographic determination of urinary 5-hydroxyindole-3-acetic acid.

The popular colorimetric assay for urinary 5-hydroxyindole-3-acetic acid based on the reaction with 1-nitroso-2-naphthol has been criticized for lack of specificity. Goldenberg (Clin. Chem. 19: 38-44, 1973) proposed a modification of this method, in which 2-mercaptoethanol is used to discharge unwanted chromophores. We compared results by both the unmodified and modified colorimetric methods wi...

Does elevated urinary 5-hydroxyindole acetic acid level predict acute appendicitis in children?

BACKGROUND Acute appendicitis is the most common abdominal surgical emergency in children, and appendectomy is the most frequent acute abdominal operation. Prompt diagnosis and surgical treatment are required to reduce the risk of perforation and prevent complications, especially in small children. Enterochromaffin cells that contain large amounts of serotonin are mostly located in the distal a...

Simple determination of high urinary excretion of 5-hydroxyindole-3-acetic acid with ferric chloride.

We describe a method for determination of urinary 5-hydroxyindole-3-acetic acid, a metabolite of serotonin and tryptophan and a useful indicator of metastasizing carcinoid tumor. The analysis is based on the color development with ferric chloride added to the residue obtained on evaporating a diethyl ether extract of the sample that has been acidified with sulfuric acid and saturated with sodiu...

Association between urinary albumin excretion and plasma 5-hydroxyindole-3-acetic acid concentration in men with type 2 diabetes.

S erotonin (5-hydroxytryptamine [5-HT]) mediates vasoconstriction and induces the activation of platelets, which may promote atherosclerosis. Plasma 5-HT concentrations have been reported to be high in diabetic patients (1,2); this may be one of the underlying mechanisms of diabetes complications. The 5-HT2A receptor has been identified in glomerular mesangial cells (3), suggesting involvement ...